Analgetik Power Test Of Herba Katuk (Sauropus androgynus) Ethanol Extract On Mice (Mus musculus) With Acetic Acid Induction
UJI EFEKTIVITAS ANALGETIK EKSTRAK ETANOL HERBA KATUK (Sauropus androgynus) PADA MENCIT PUTIH (Mus musculus) DI INDUKSI ASAM ASETAT
DOI:
https://doi.org/10.48144/jiks.v15i2.1183Keywords:
katuk herb, Sauropus androgynus, analgesic, stretching methodAbstract
. Indonesia is a country with an abundance of potential natural resources. Herbaceous plants, which are found in forests and are smaller than trees or shrubs, have moist stems, and are not woody, are one type of plant utilized in traditional medicine. Katuk is a herbaceous plant that belongs to the Euphorbiaceae family. This study aims to examine the analgesic effectiveness of katuk herb (Sauropus androgynus) in mice (Mus musculus) compared to diclofenac sodium.
The method used for the analgesic test is the stretching method. Twenty-five mice were separated into five groups, each with five mice in a row, namely positive control (diclofenac sodium), negative control (Na-CMC 0,5%), dose 1 (8.4 mg/20 g BW), dose 2 (11.2 mg/20 g BW), and dose 3 (14 mg/20 g BW). Each group was administered the test material orally, then 1 percent acetic acid intraperitoneally 30 minutes later, and the number of stretches was counted every 15 minutes for 90 minutes.
The results obtained were analyzed using one way ANOVA test. The test results revealed that the higher the dose of katuk herbs given, the better the analgesic effect. Based on the ANOVA test and LSD test, katuk herb at dose 3 had a higher effectiveness than the positive control and the treatment group at dose 1, and dose 2.
References
Al-Muqsith, A. (2015). Luka (Vulnus). Fakultas Kedokteran, Universitas Malikussaleh.
Castillo, M, A., & Bruzzone, L. (2006). Indirect Fluorometric Determination of Diclofenac Sodium. Analytical Sciences, 22, 431–433.
Dalimartha, S. (2000). Atlas Tumbuhan Obat Indonesia ((jilid II) ed). Jakarta: Trubus Agriwidia.
Galani, V. J., & Patel, B. . (2011). Analgesic and Anti-inflammatory Activity of Argyreia spesiosa and Sphearanthusn indicus in the Experimental Animal. Global Journal of Pharmacology, 5, 54–59.
Ganie, S. (2003). Upaboga di Indonesia. Jakarta: PT Grafika Multiwarna.
Gawade, S. (2012). Acetic Acid Induced Painful Endogenous Infliction in Writhing Test on Mice. Journal of Pharmacology and Pharmacotherapeutics, 3(4), 348.
Goenarwo, Edijanti, & Susanto. (2011). Uji Efektivitas Analgetik Madu Pada Tikus Dengan Metode Geliat Asetat Studi Eksperimental Pada Tikus Putih Jantan Galur Wistar. Sains Medika, 3(1), 48–53.
Gunawan, S. . (2008). Farmakologi dan Terapi ed 5, Jakarta : Balai Penerbit FKUI.
Istiqomah. (2013). Perbandingan Metode Ekstraksi Maserasi Sokletasi Terhadap Kadar Piperin Buah Cabe Jawa (Piperis retrofracti fructus). Skripsi Jurusan Farmasi UIN Syarif Hidayatullah Jakarta.
Kudaravalli, J., D, N., & Vijayalakhsmi. (2011). Efficacy and Safety of Diclofenac Sodium and Aceclofenac in Controlling Post Extraction Dental Pain: A Randomized Open Label Comparative Study. J Pharmacol Toxicol, 6(5), 541–547.
Kusriani, R., H., Rahmawati, I., & Musfiroh, I. (2014). Karakterisasi Pati Biji Buah Durian, Biji Buah Nangka, dan Biji Buah Alpukat. Jurnal Farmasi Galenika, 1(1).
Puspitasari, H., Listyawati, Shanti, & Tetri, W. (2003). Aktivitas analgetik ekstrak umbi teki (Cyperus rotundus L.) pada mencit putih (Mus musculus L.) jantan. Biofarmasi, 1.2, 50–57.
Putra. (2014). Ekstraksi Zat Warna Alam dari Benggol Tanaman Pisang (Musa Paradisiaca L.) dengan Metode Maserasi, Refluks dan Sokletasi. Jurnal Kimia, 8(1), 113–119.
Seidel, V. (2008). Initial and Bulk Extraction. In: Sarker, S. D., Latif, Z. and Gray, A. I., editors. Natural Products Isolation. 2nd Ed. New Jersey: Humana Press, 33–34.
Susanti, N., Budiman, I., & Warditiani, N. . (2014). Skrining Fitokimia Ekstrak Etanol 90% Daun Katuk (Sauropus androgynus). Bali.
Thompson, E. B. (1985). Drug joscreening fundamental of drug evaluation technique in pharmacology. New York Publishing Co, Inc.
Tjay, T., & Raharja, K. (2007). Obat-Obat Penting : Khasiat, Penggunaan dan Efek Samping (Edisi IV ed.). PT. Elex Media Komputindo.
Triswanto, S., & Susiyanto, P. (2016). Uji Efek Analgesik Ekstrak Etanol Daun Kersen (Muntingia calabura L.) Pada Mencit Putih Jantan (Mus musculus) Dengan Induksi Nyeri Asam Asetat. Farmasi Samarinda: Jurnal Ilmiah Manuntung.
Turner, R. (1965). Screening Methods In Pharmacology. Academi Press. New York.
Victor Owoyele, B., Oguntoye, S. O., Dare, K., Ogunbiyi, B. A., Aruboula, E. A., & Soladoye, A. O. (2008). Analgesic, anti-inflammatory and antipyretic activities from flavonoid fractions of Chromolaena odorata. Journal of Medicinal Plants Research, 2(9), 219–225. http://www.academicjournals.org/JMPR
Wahyuni, T. L., Astuti, Y., & Nuratmi, B. (2012). Uji Perbandingan Efek Analgesik Infus Temu Putih (Curcuma Zedoaria Rosc.) Dan Temu Mangga (Curcuma Mangga Val. Et Zipp) Pada Mencit. Jurnal Bahan Alam Indonesia, 2(3), 81–84.
